bladder cancer t24 cells  (Procell Inc)

Journal: Frontiers in Immunology

Article Title: Ribosome biogenesis programs define a three-gene RBscore with prognostic relevance in bladder cancer

doi: 10.3389/fimmu.2026.1810132

Figure Lengend Snippet: Experimental validation of RBscore signature gene expression. (A) RT-qPCR of PIN4, POP4, and PRKDC mRNA in SV-HUC-1 and T24 cells. (B) RT-qPCR of PIN4, POP4, and PRKDC mRNA in paired BLCA and adjacent normal tissues. (C) Western blot analysis and quantification of PIN4, POP4, and PRKDC protein in SV-HUC-1 and T24 cells. (D) Representative IHC images of PRKDC in BLCA and adjacent normal tissues. (E) Quantification of PRKDC IHC scores in BLCA and adjacent normal tissues. * P < 0.05, *** P < 0.001. BLCA, bladder cancer; RBscore, ribosome biogenesis related score; IHC, immunohistochemistry.

Article Snippet: Human bladder urothelial SV-HUC-1 cells (Procell, China) and bladder cancer T24 cells (Procell, China) were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: BMC Cancer

Article Title: The potential antitumor effects of combining intravesical therapy with recombinant bacillus calmette-guérin and an anti-PD-1 inhibitor in bladder cancer

doi: 10.1186/s12885-026-15890-x

Figure Lengend Snippet: Cell viability of 3D bioprinted bladder cancer-on-a-chip model. A Live/dead staining on the 3rd day after drug treatment. T24 bladder cancer cell blocks in the rBCG- dltA and pembrolizumab combination group had lower cell survival rates than those in the other groups did. Noncancerous cell lines, such as HUVECs and MRC-5 cells, survived well in all groups. The scale bar at the lower right corner represents 200 μm. B According to the results of the quantitative cell density test, T24 cancer cells were affected by rBCG- dltA and pembrolizumab. The group with the lowest cell density was the combination-treated group. * p < 0.05 vs. control

Article Snippet: The 3D bioprinted BCOC blocks consisted of the T24 bladder cancer cells, MRC-5 fibroblasts, Jurkat T lymphocytes, THP-1 monocytes, and human umbilical vein endothelial cell line cells (HUVEC), cultured using standard protocols provided by the suppliers: Korean Cell Line Bank (Seoul, Republic of Korea) and Lonza (Basel, Switzerland).

Techniques: Staining, Control

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Representative haematoxylin–eosin (HE)-stained sections of SV-HUC-1 and T24 spheroids prepared by cryosectioning ( A-F ) and paraffin embedding (G-L) . A necrotic core (indicated by asterisks, A-B , D-E , G-H , J-K ) is visible in most cross sections, except those obtained from peripheral regions. Scale bars: 500 µm (A, D, G, J), 100 µm (B, E, H, K), 50 µm (C, F, I, L).

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Staining

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Representative images of paraffin sections show E-cadherin (green) in the plasma membrane in cells of SV-HUC-1 spheroids ( A-a3 ) and N-cadherin (red) in the plasma membrane of T24 spheroids ( B-b3 ). Some SV-HUC-1 cells are also positive for N-cadherin (arrows, a2 and a3 ). Yellow insets (in A and B) are magnified (a1-a3 and b1-b3) and display individual and merged channels of E-cadherin, N-cadherin, and DAPI-stained nuclei. Scale bars: 100 µm (A, B) , 20 µm (a1-a3, b1-b3).

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Clinical Proteomics, Membrane, Staining

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: SV-HUC-1 (A) and T24 cells (C) form spheroids with a spherical morphology. (B) SV-HUC-1 cells are tightly attached to each other (arrows), and microvilli are seen on their surface. (D) T24 cells are loosely attached, displaying wider intercellular spaces (arrowheads) and have fewer microvilli. Scale bars: 100 µm (A, C) , 10 µm (B, D) .

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques:

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Outermost cells in SV-HUC-1 spheroids display cuboidal morphology (A) , whereas T24 cells are more elongated (B) . The presence of cell junctions in the outermost cell layer of SV-HUC-1 (C) and T24 spheroid (D) (boxed regions) indicates a tight cellular network of the outermost cell layer. The central necrotic zone (asterisks) is filled with necrotic cells in SV-HUC-1 and T24 spheroids (E, F) , as clearly demonstrated on semithin sections (G, H) , prepared before ultrathin sectioning. Scale bars: 100 µm (G-H) , 10 µm (A-B) , 5 µm (E-F) , 1 µm (C-D) .

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: